Mastitis Diagnostic Methods Comparison: CMT, PCR, Flow Cytometry
| Method | Principle | Sensitivity | Time | Cost/test | Use case |
|---|---|---|---|---|---|
| CMT | Chemical reagent reacts with SCC | Low (qualitative only) | 2 min | US $0.5 | On-farm screening |
| Flow-cytometry SCC | Fluorescent staining + flow count | High (±5 % error) | 5 min/sample | US $1 | Milk-plant QC lab |
| Microbial culture | Selective media isolation | Medium (sample-dependent) | 48–72 h | US $8 | ID + antibiogram |
| Real-time PCR | Target DNA amplification & fluorescence | Very high (1–10 CFU) | 2 h | US $15 | Rapid multi-pathogen ID |
| Nanopore sequencing | Direct pathogen genome sequencing | Comprehensive | 6 h | US $50 | Complex or unknown infections |
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