Multiplex Molecular Diagnostic Solutions for Dairy Cow Mastitis
Multiplex real-time PCR:
-
Example: simultaneous detection of S. aureus (nuc), Streptococcus (sodA), E. coli (phoA) with 50 CFU/mL sensitivity.
-
Commercial kit: Thermo Fisher VetMAX™ Gold BVP Panel (8 pathogens).
CRISPR-Cas assay:
-
Principle: Cas12a cleaves fluorescent reporter; result in 1 h (e.g., MammiCheck system).
-
Pros & cons:
– Multiplex PCR: high throughput, risk of primer interference.
– CRISPR: no complex instrument, but requires pre-amplification.
Stay tuned for WISEBIO’s rapid multiplex PCR mastitis panel…
Specimena Bloka Citaĵo
Nam tempus turpis at metus scelerisque placerat nulla deumantos sollicitudin delos felis. Pellentesque diam dolor an elementum et lobortis at mollis ut risus. Curabitur sempre sagittis mino de condimentum.
Ekzempla Paragrafa Teksto
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi ut blandit risus. Donec mollis nec tellus et rutrum. Orci varius natoque de penatibus et magnis dis parturient montes, nascetur ridiculus mus. Ut consequat quam a purus faucibus scelerisque. Mauris ac dui ante. Pellentesque congue porttitor tempus. Donec sodales dapibus urna sed dictum.